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  • daysfoot
    Junior Member
    • Aug 2013
    • 6

    test for bio-differences with RNA-seq

    The test of the differences between two biology replicate based on RNA-Seq data is really bad.

    By cuffdiff, there are 5600 different significant expressed genes in gene_exp.diff.

    I can not figure out the reason?

    Do you have the same problem ever?
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    It sounds like you're wondering if one of your samples is an outlier. It's usually easiest to tell that sort of thing in a PCA graph (though you'll often have an idea beforehand when you do QC).

    Comment

    • chadn737
      Senior Member
      • Jan 2009
      • 392

      #3
      Why is that bad? Depending on what the samples are, I do not find that unusual at all.

      Comment

      • GenoMax
        Senior Member
        • Feb 2008
        • 7142

        #4
        Wording in original posting seems to indicate that this may be a comparison of two (single) biological samples (replicates). But I may be wrong.

        Comment

        • chadn737
          Senior Member
          • Jan 2009
          • 392

          #5
          Originally posted by GenoMax View Post
          Wording in original posting seems to indicate that this may be a comparison of two (single) biological samples (replicates). But I may be wrong.
          If that is the case, then I would not consider looking for DE, especially by cuffdiff, to be at all a valid means of determining how similar/dissimilar the samples are. Particularly if the samples are not genetically homogeneous, etc.

          Comment

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