Sequencing cDNA 5' ends

rnaseq0814

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Join Date: Sep 2013

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Sequencing cDNA 5' ends

09-19-2013, 02:09 PM

Hi,

I'm trying to develop a protocol where I ligate on an adapter at the 5' end of a CDNA, but I want to make sure that in my sequencing reads that I always see the 5' end to make sure I can include this data (ideally want to sequence everything with a 5' adapter). Does anyone have any experience with trying to develop a protocol to do this on the Illumina platform?

Thanks!
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