Recently sequenced a ribo-reduction library on an Illumina HiSeq. FASTQC analysis showed an unusual spiky per_sequence_GC_content distribution (see attached). There was an overrepresented sequence that blasts to a snoRNA. I'm wondering if anyone else has seen this unusual spiky GC content distribution with a ribo-reduction library.
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM