Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • KnowNothing2
    Member
    • Sep 2013
    • 49

    #1

    Does these Cuffcompare results look okay

    I aligned my RNAseq data to the mouse mm10 genome using Bowtie2. I then generated a GTF file using Cufflinks, and then used Cuffcompare to compare against an annotated mouse genome (from UCSC Table Browswer).

    # Cuffcompare v2.1.1 | Command line was:
    #/home/adam/cufflinks/cuffcompare transcripts.gtf -r refFlatMouse.gtf
    #

    #= Summary for dataset: transcripts.gtf :
    # Query mRNAs : 13129 in 13129 loci (0 multi-exon transcripts)
    # (0 multi-transcript loci, ~1.0 transcripts per locus)
    # Reference mRNAs : 30786 in 23311 loci (27576 multi-exon)
    # Corresponding super-loci: 3150
    #--------------------| Sn | Sp | fSn | fSp
    Base level: 2.5 68.1 - -
    Exon level: 0.1 2.3 1.2 20.3
    Intron level: 0.0 -nan 0.0 -nan
    Intron chain level: 0.0 -nan 0.0 -nan
    Transcript level: 0.0 0.0 0.0 0.0
    Locus level: 0.0 0.0 0.0 0.0

    Matching intron chains: 0
    Matching loci: 0

    Missed exons: 206087/216519 ( 95.2%)
    Novel exons: 2718/13129 ( 20.7%)
    Missed introns: 193970/193970 (100.0%)
    Missed loci: 20087/23311 ( 86.2%)
    Novel loci: 2718/13129 ( 20.7%)

    Total union super-loci across all input datasets: 5887

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
23 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
27 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-13-2026, 09:56 AM
0 responses
23 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-09-2026, 11:14 AM
0 responses
28 views
0 reactions
Last Post SEQadmin2  
Working...