Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Does these Cuffcompare results look okay

    I aligned my RNAseq data to the mouse mm10 genome using Bowtie2. I then generated a GTF file using Cufflinks, and then used Cuffcompare to compare against an annotated mouse genome (from UCSC Table Browswer).

    # Cuffcompare v2.1.1 | Command line was:
    #/home/adam/cufflinks/cuffcompare transcripts.gtf -r refFlatMouse.gtf
    #

    #= Summary for dataset: transcripts.gtf :
    # Query mRNAs : 13129 in 13129 loci (0 multi-exon transcripts)
    # (0 multi-transcript loci, ~1.0 transcripts per locus)
    # Reference mRNAs : 30786 in 23311 loci (27576 multi-exon)
    # Corresponding super-loci: 3150
    #--------------------| Sn | Sp | fSn | fSp
    Base level: 2.5 68.1 - -
    Exon level: 0.1 2.3 1.2 20.3
    Intron level: 0.0 -nan 0.0 -nan
    Intron chain level: 0.0 -nan 0.0 -nan
    Transcript level: 0.0 0.0 0.0 0.0
    Locus level: 0.0 0.0 0.0 0.0

    Matching intron chains: 0
    Matching loci: 0

    Missed exons: 206087/216519 ( 95.2%)
    Novel exons: 2718/13129 ( 20.7%)
    Missed introns: 193970/193970 (100.0%)
    Missed loci: 20087/23311 ( 86.2%)
    Novel loci: 2718/13129 ( 20.7%)

    Total union super-loci across all input datasets: 5887

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 08:47 AM
0 responses
12 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
60 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
60 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
54 views
0 likes
Last Post seqadmin  
Working...
X