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  • AJenkins
    Member
    • Nov 2013
    • 13

    #1

    Cufflinks Killed Before Complete

    I am attempting a RABT assembly of a genome which contains contigs 2L,2R,3L,3R,X, Y_unplaced and UNKN. Cufflinks inspects bundles for each of the 7 contigs provided from the GTF file but when it moves onto the next step of assembling transcripts and estimating abundances, it only gets through 2L, 2R, 3L and most of 3R and then I receive this statement:

    Code:
    "Reduced 28 frags to 23 (17.857143 percent)
    Calculating intial MLE
    Convergence reached in 19 iterations 
    Tossing likely garbage isoforms
    Filtering isoform 43210387-43212162
    Revising MLE
    Convergence reached in 17 iterations 
    Killed"
    Is there a reason why the program may have been killed? I'm not sure why this happens. I've checked the resulting output transcrits.gtf file and there are only 2L,2R,3L and 3R. None of the other contigs are mapped. Any ideas?
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    It was killed by the operating system. Perhaps it was using too much memory. Check your system logs.

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