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I would like to calculate raw counts for each cufflinks transcript. I can convert to reads per million by multiplying by the length of the transcript, but I'm not sure how what the cufflinks software uses as the total number of reads. Is it the number of unique reads in the sam input file? Thank you!
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Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has seen remarkable advancements,...12-02-2024, 01:49 PM