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Duplicates and 3' RNA sequencing of FFPE samples

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  • Duplicates and 3' RNA sequencing of FFPE samples

    Hi everyone
    I am currently working on RNA sequencing of poor quality formalin fixed samples
    I am using the Lexogen quantseq Kit sith sequencing using a Novaseq
    I am getting a very high percentage of duplicates that may be more than 90%
    But I think as we only sequence the end of transcripts, it is not overall not very surprising to have high rates of identical sequences? Do anyone have any feedback on this?
    Thanks+++
    Julien
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