Hi everyone
I am currently working on RNA sequencing of poor quality formalin fixed samples
I am using the Lexogen quantseq Kit sith sequencing using a Novaseq
I am getting a very high percentage of duplicates that may be more than 90%
But I think as we only sequence the end of transcripts, it is not overall not very surprising to have high rates of identical sequences? Do anyone have any feedback on this?
Thanks+++
Julien
-
This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.
The Headliner
The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...03-03-2025, 01:39 PM -
The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...02-24-2025, 06:31 AM