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  • scienceguy
    Administrator
    • Dec 2021
    • 5

    Article - Streamlining the RNA Extraction and Purification Workflow


    ​​Any research project that ostensibly starts with a sample of purified RNA—such as RNA-seq and RT-PCR—inevitably begins earlier, with the process of obtaining the RNA from tissue or isolated cells. But extracting and purifying RNA from that important starting sample is no easy task. RNA is an unstable molecule that easily degrades unless care is taken to preserve it during the process of extraction. Further purification removes contaminants, DNA, and other unwanted molecules to obtain high-quality RNA samples needed for the best results in downstream applications. This article looks at optimized reagents, kits, and workstations that researchers rely on to streamline workflows for RNA extraction and purification.

    ow.ly/KvEr50JoaPh
    Last edited by scienceguy; 06-11-2022, 12:31 PM.

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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