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Article - Streamlining the RNA Extraction and Purification Workflow
Any research project that ostensibly starts with a sample of purified RNA—such as RNA-seq and RT-PCR—inevitably begins earlier, with the process of obtaining the RNA from tissue or isolated cells. But extracting and purifying RNA from that important starting sample is no easy task. RNA is an unstable molecule that easily degrades unless care is taken to preserve it during the process of extraction. Further purification removes contaminants, DNA, and other unwanted molecules to obtain high-quality RNA samples needed for the best results in downstream applications. This article looks at optimized reagents, kits, and workstations that researchers rely on to streamline workflows for RNA extraction and purification.
ow.ly/KvEr50JoaPhLast edited by scienceguy; 06-11-2022, 12:31 PM.
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The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i
...01-24-2023, 01:19 PM -
Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.
The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes...01-09-2023, 03:10 PM