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Article - Streamlining the RNA Extraction and Purification Workflow
Any research project that ostensibly starts with a sample of purified RNA—such as RNA-seq and RT-PCR—inevitably begins earlier, with the process of obtaining the RNA from tissue or isolated cells. But extracting and purifying RNA from that important starting sample is no easy task. RNA is an unstable molecule that easily degrades unless care is taken to preserve it during the process of extraction. Further purification removes contaminants, DNA, and other unwanted molecules to obtain high-quality RNA samples needed for the best results in downstream applications. This article looks at optimized reagents, kits, and workstations that researchers rely on to streamline workflows for RNA extraction and purification.
ow.ly/KvEr50JoaPhLast edited by scienceguy; 06-11-2022, 12:31 PM.Tags: None
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by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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