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  • Article - Streamlining the RNA Extraction and Purification Workflow


    ​​Any research project that ostensibly starts with a sample of purified RNA—such as RNA-seq and RT-PCR—inevitably begins earlier, with the process of obtaining the RNA from tissue or isolated cells. But extracting and purifying RNA from that important starting sample is no easy task. RNA is an unstable molecule that easily degrades unless care is taken to preserve it during the process of extraction. Further purification removes contaminants, DNA, and other unwanted molecules to obtain high-quality RNA samples needed for the best results in downstream applications. This article looks at optimized reagents, kits, and workstations that researchers rely on to streamline workflows for RNA extraction and purification.

    ow.ly/KvEr50JoaPh
    Last edited by scienceguy; 06-11-2022, 12:31 PM.

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  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

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