RNA variants

[Rkn0068]

Hi,

I am new to this forum and also to NGS data analysis. May I know if it is possible to analyse RNA variants from whole transcriptome/RNA sequencing (single end reads)? If so, I would appreciate if you could suggest appropriate literature/tools to carry out the analysis.

Thank you.

[César]

Hi. Also, I´m new in this kind of analysis. Even I don´t know if there is exist a code in R-studio or a comand for a terminal to automate this analysis of variants or isoforms, you can use the software integrative genomic viewer (IVG) for watch the alternative splicing through the sashimi plot tool. You need to have your RNA-seq file in a bam format. Then, you can downloaded in the software IVG together with your reference genome and apply the sashimi plot.