Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Need help to get a DEseq or EdgeR example file

    Hi,
    I am a novice in RNA-seq data analysis.

    I need a list of DEG from RNA-seq data. I need to test this list against some new workflows developed inhouse. Can anyone suggest how i can get such a list.

    I tried GEO website and uploaded the files from the GSE44674 dataset.

    GSE44674_DESeq_normalised_matrix_file_all_55_samples.txt.gz and
    GSE44674_TKT-TMM-EDGER-NORM-55-SAMPLES.txt.gz

    I presumed that since these are output files from EDGER and DEseq, they should have a list of DEG with p-values and log fold change.

    On the contrary these files have columns for GO accession and columns for all 55 samples with values.

    How can i find a list of DEG analyzed from RNA-seq data?
    Any help in this direction will be very helpful.

    Thanks and Regards,
    Neetu

Latest Articles

Collapse

  • seqadmin
    Choosing Between NGS and qPCR
    by seqadmin



    Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
    10-18-2024, 07:11 AM
  • seqadmin
    Non-Coding RNA Research and Technologies
    by seqadmin




    Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.

    Nobel Prize for MicroRNA Discovery
    This week,...
    10-07-2024, 08:07 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 11-01-2024, 06:09 AM
0 responses
12 views
0 likes
Last Post seqadmin  
Started by seqadmin, 10-30-2024, 05:31 AM
0 responses
14 views
0 likes
Last Post seqadmin  
Started by seqadmin, 10-24-2024, 06:58 AM
0 responses
24 views
0 likes
Last Post seqadmin  
Started by seqadmin, 10-23-2024, 08:43 AM
0 responses
52 views
0 likes
Last Post seqadmin  
Working...
X