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  • Tali7
    Junior Member
    • Aug 2011
    • 8

    #1

    RNA extraction -QIAGEN's Buffer RLT followed by organic extraction protocol?

    Hi guys. I have some tissue homogenates in QIAGEN's Buffer RLT+, stored in the -80 freezer. I want to extract some RNA from these samples for downstream NGS applications, and was wondering if it's possible to follow an organic extraction method for samples in RTL+?

    I normally homogenise in TRIreagent which is fine, but i can't find the molecular composition of RLT+ anywhere! Is it best to use an RNeasy column or similar to extract my RNA from these homogenates?

    Thanks very much

    Tali

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