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  • mtiwaridros
    Member
    • Feb 2014
    • 20

    #1

    Genome generation with STAR

    Hi
    I want to generate a genome using STAR for subsequent steps.
    I got the genome.fa file from UCSC for my organism (dm).
    I ran:

    Code:
    /home/STAR --runMode genomeGenerate --genomeDir /media/star_pipe --genomeFastaFiles genome.fa /media/star_pipe runThread 4
    However it has been 15 h now and it is stuck at:
    Code:
    Starting to generate Genome files
    Does it usually take this long or am I doing something wrong?
    I have a quad-core CPU with 16 GB RAm, and do not see any unsusal SWAP activity. The CPU usage for STAR is 25-30%.

    Any suggestions/corrections will be appreciated.

    Thanks.
    Manu
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    See this: http://seqanswers.com/forums/showthread.php?t=31815

    You may have the runThread option incorrectly specified, should be "--runThreadN 4"

    Have you checked to see if the index files are increasing in size?

    Comment

    • mtiwaridros
      Member
      • Feb 2014
      • 20

      #3
      Hi GenoMax

      I figured it out.
      And its the same as you pointed.

      Now I have my built genome file.

      Thanks.

      Best
      Manu

      Comment

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