Hi there,
I'm planning to do some miRNAseq on human FFPE tissue. I've used the Qiagen FFPE miRNA kit to extract goal RNA including miRNA. On quantifying the RNA I get a big disparity between the Qubit concentration and the Agilent tape station concentration - why is this and which is more reliable. I used to do a Nanodrop concentration as well but found with previous DNA/RNA work that it always overestimated the concentration and the contamination ratios made no difference to the quality of sequencing library I made in the past so I don't bother with it anymore.
Any advice?
I'm planning to do some miRNAseq on human FFPE tissue. I've used the Qiagen FFPE miRNA kit to extract goal RNA including miRNA. On quantifying the RNA I get a big disparity between the Qubit concentration and the Agilent tape station concentration - why is this and which is more reliable. I used to do a Nanodrop concentration as well but found with previous DNA/RNA work that it always overestimated the concentration and the contamination ratios made no difference to the quality of sequencing library I made in the past so I don't bother with it anymore.
Any advice?
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