15 results in 0.0021 seconds.
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Preferred analyzer for library size/library quality
Hello! I am new to the world of NGS and am curious about the preferred instrument for checking library quality. The ones that seem most popular are Agilent... -
Libraries - Unusual Profiles on Tapestation
Hello,
I'm posting this hoping that you can help me understanding 3 unusual Tapestation Profiles. These profiles correspond to (DNA) libraries... -
bioanalyzeR: New R package for data from the Agilent Bioanalyzer and TapeStation
I wrote an R package for importing electrophoresis data from the Agilent Bioanalyzer and TapeStation and graphing the results with ggplot2 or analyzing... -
Tapestation imaging
How does the Tapestation (2200/4200) measure fluorescently stained biomolecules ?
Does it scan fluorescence in real-time (during eletrophoresis)... -
Looking for a standardized method of library quantification and quality control
Hello all,
I am wondering if anyone out there has discovered/created a standardized method for library quantification and quality control... -
Bioanalyzer/TapeStation fragment sizes larger than agarose gel
I am preparing libraries for GBS, and although the agarose gel image is as expected (i.e. the bulk of fragments between 200 and 400bp), TapeStation result...Last edited by cpylant; 10-27-2017, 08:56 AM. -
Amplicon resolving differently between bioanalyzer and tapestation
Hello,
We are seeing a discrepancy between the Bioanalyzer and Tapestation for a large amplicon product that we received for sequencing. We received...Last edited by Pab77; 01-25-2016, 12:13 PM. -
TRIzol contamination in low yield RNA extraction.
SCENARIO:
- Goal: RNA-seq.
- Number of samples (already extracted): 200.
- Size of individual sample: VERY small (single zebrafish... -
TapeStation 4200
Has anyone here tried the new Agilent's device, TapeStation 4200?
http://www.genomics.agilent.com/en/T...eStation-Instr... -
Tapestation quality peaks show unwanted larger fragments.
Hi all,
I used Covaris to shear human genomic DNA at a set size of 300bp and 200bp respectively. This is to sequence my samples...Last edited by mzahir89; 08-19-2015, 02:20 AM. -
Agilent TapeStation vs. Qubit: RNA concentration.
SCENARIO:
After RNA extraction (RNA-seq is my aim), I check the concentration of the extracted RNA with Qubit, and then run the extracted... -
TapeStation 2200: multiple problems
Dear users,
this is my first post at SEQanswers. I work at a NGS core facility as scientific head, which involves a lot of troubleshooting.... -
Problems with Agilent Tapestation R6K Screentapes
Hi Everyone,
We've had a Tapestation 2200 for a while, but recently we've been having problems with the R6K screen tapes (squashed bands,... -
Which is more reliable - Tapestation or Qubit?
Hi there,
I'm planning to do some miRNAseq on human FFPE tissue. I've used the Qiagen FFPE miRNA kit to extract goal RNA including miRNA.... -
Agilent TapeStation 2200?
Anyone tried one as a bioanalyzer replacement? 1-16 samples, vague but seems to load itself from strips or plates , can use partial chips. Not quite...
