Would RNA quality/quantity be good enough for sequencing when the liver tumor samples

[woodydon]

Dear all,

Would RNA quality/quantity be good enough for sequencing when the liver tumor samples are frozen and sliced?

One of my colleague thinks that the RNA quality should be fine for those cryo-sliced liver tumor samples. We just need to use more sliced samples to get enough RNA. Is this correct?

One of my friends told me that the sliced liver tumor samples will have poor RNA quality, so I am worried. I am a totally dry lab geek. Could please someone help me? Thanks!

Woody

[dpryan]

The only way to find out would be to extract RNA from a bunch of slices and have a look on a bioanalyzer. I've sequenced RNA from frozen liver samples without issue, so unless the cryo-slicing wasn't really cryo enough then I would expect it'd work.

[woodydon]

The only way to find out would be to extract RNA from a bunch of slices and have a look on a bioanalyzer. I've sequenced RNA from frozen liver samples without issue, so unless the cryo-slicing wasn't really cryo enough then I would expect it'd work.

Hi dpryan,

Thanks!

Woody