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  • Adapter trimming

    Hi

    I have got the adapter sequences from company as for Illumina RNA seq data:
    5-AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGACGATC-(insert)-ACCTTAAGAGCCCACGGTTCCTTGAGGTCAGTGXXXXXXTAGAGCATACGGCAGAAGACGAAC-3

    (ID #1 "ATCACG" )

    Will this be my adapter sequences to trim it using trimmomatic?
    >adpter1
    AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGACGATC
    >adapter2
    ACCTTAAGAGCCCACGGTTCCTTGAGGTCAGTGATCACGTAGAGCATACGGCAGAAGACGAAC

    or I have to reverse complement the adapter ?

    Best Regards

  • #2
    Have a look at the TruSeq adapter files that come with trimmomatic,
    and see how they compare to the sequences you have.

    Comment


    • #3
      what does these adaptors have in common?

      Comment

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