could not find function "DESeqDataSetFromMatrix"

[mtiwaridros]

Hi

I am trying to use DESeq2 for differential expression analysis.
Everything goes fine:
DESeq2 is installed in R-

.......
installing to /usr/local/lib/R/site-library/DESeq2/libs
** R
** inst
** preparing package for lazy loading
** help
*** installing help indices
** building package indices
** testing if installed package can be loaded
* DONE (DESeq2)

but upon using DESeqDataSetFromMatrix, I get the error:

Error: could not find function "DESeqDataSetFromMatrix"

The command that I am giving is:

Data1<-DESeqDataSetFromMatrix(countData=countData,colData=colData, design=~condition)

Any clues/help shall be much appreciated.

Thanks.
M

[dpryan]

You forgot to:

library(DESeq2)

Just because it's installed doesn't mean it's loaded.

[mtiwaridros]

Hi dpryan

Yes, I already did library(DESeq2).
But now I figured out the problem, which was due to RStudio.
It is working fine in the shell.
However, I thank you very much for taking time out and pointing out these noob mistakes.

Best
M