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Hello,
I have paired end Illumina sequences s_1_1_sequnece.txt and s_1_2_sequence.txt. These sequence files included indexed and nonindexed reads. When I tried to separate the sequence file one by one, I found the numbers of sequences in indexed and nonindexed files for one of the pair are different from the numbers for the other of the pair. So I need scripts to open the two files and check the pattern matching the reads in both files and pick them out from both files.
My scripts to open to files got memory problem.I am not good at perl. I am wondering who has any easy or efficiency way to separate the indexed and nonindexed sequences for both of paired sequence files together?
Any suggestions or comments are absolutely appreciated.
Lisa
I have paired end Illumina sequences s_1_1_sequnece.txt and s_1_2_sequence.txt. These sequence files included indexed and nonindexed reads. When I tried to separate the sequence file one by one, I found the numbers of sequences in indexed and nonindexed files for one of the pair are different from the numbers for the other of the pair. So I need scripts to open the two files and check the pattern matching the reads in both files and pick them out from both files.
My scripts to open to files got memory problem.I am not good at perl. I am wondering who has any easy or efficiency way to separate the indexed and nonindexed sequences for both of paired sequence files together?
Any suggestions or comments are absolutely appreciated.
Lisa