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We are planing a miRNA sequencing for our mouse muscle samples.
Usually, we get very good RNA samples with our Qiagen miRNeasy kit, with RIN >8.
Unfortunatelly, for this batch, we have RIN ~5.0 for all samples.
A260/A280 is always around 2.0, which excludes contamination. The tissue is freshly dissected and stored in RNAlater at -20 C for 2 days. Then we extracted RNA using Qiagen miRNeasy kit.
It is some weird that we have such low RIN numbers.
Now, we need make a choice, to throw these RNA samples away or go forward for microRNA sequencing?
Anyone has similar experience?
Advice is appreciated.
Usually, we get very good RNA samples with our Qiagen miRNeasy kit, with RIN >8.
Unfortunatelly, for this batch, we have RIN ~5.0 for all samples.
A260/A280 is always around 2.0, which excludes contamination. The tissue is freshly dissected and stored in RNAlater at -20 C for 2 days. Then we extracted RNA using Qiagen miRNeasy kit.
It is some weird that we have such low RIN numbers.
Now, we need make a choice, to throw these RNA samples away or go forward for microRNA sequencing?
Anyone has similar experience?
Advice is appreciated.