storing TruSeq libraries > 1 week

[saw9]

Hello all,

I recently had to travel unexpectedly and have had to keep some TruSeq stranded libraries in the -20 for more than the 1 week called for in the Illumina protocol. They're at 2 weeks now but have not yet been diluted to 10nm. I have more libraries to finish this week and hope to send them for sequencing by week's end. I'm inclined to believe that undiluted libraries are still OK for a few weeks but thought I'd ask for good measure. Thanks in advance!

[nucacidhunter]

They should be fine. The libraries in double strand form are like any other DNA and store well at -20C. I have sequenced libraries stored for several months. Issues can raise when low concentrated normalised libraries are stored and clustered without re-quantification.