Hi, I'm new to RNA-Seq and am looking for some information on the quality/size profile of RNA needed to produce high-quality data on either the 454 or Illumina platform. We are working with some environmental samples, and the goal is to sequence total prokaryotic community RNA. A MoBio soil RNA extraction kit produced decent results from an E.coli test culture but a freshly collected environmental sample looks rather degraded to my eye (see attached Bioanalyzer trace from the RNA Pico kit). I haven't found anything information on how to connect Bioanalyzer trace quality with library outcomes. Can anyone help?
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...10-30-2026, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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