Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Demultiplexinag and multiplexing

    Hi I have illumina HiSeq, base calls data and now I need to demultiplex the data.
    I am totally stuck, please provide me with the Steps and suggestions to demultiplex the data.
    Last edited by Ayyappa_kumar; 10-13-2014, 10:15 AM.

  • #2
    If this is a one-time thing why not ask the sequence provider to demultiplex the data? It is not trivial to set up the software needed to demultiplex a flowcell, especially if you don't have access to appropriate server hardware/admin expertise (alternatively you may be able to use Illumina BaseSpace to do this in the cloud).

    Comment


    • #3
      Thank you for your suggestions

      Genomax

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
        04-22-2024, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-25-2024, 11:49 AM
      0 responses
      19 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-24-2024, 08:47 AM
      0 responses
      18 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      62 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      60 views
      0 likes
      Last Post seqadmin  
      Working...
      X