Hi,
Does anyone know if it possible to improve the accuracy of Trinity RNA-Seq assemblies using --min_glue and/or --min_kmer_cov flags?
The trinity manual suggests raising the --min_kmer_cov value from the default (1) to 2 to save on memory usage and eliminate rare, inaccurate reads. Would it be advisable to raise this to higher, maybe to 4 or even to 20?
Similarly, has anyone tried raising the --min_glue to, say, 10 to improve the accuracy of the assembly?
Does anyone know if it possible to improve the accuracy of Trinity RNA-Seq assemblies using --min_glue and/or --min_kmer_cov flags?
The trinity manual suggests raising the --min_kmer_cov value from the default (1) to 2 to save on memory usage and eliminate rare, inaccurate reads. Would it be advisable to raise this to higher, maybe to 4 or even to 20?
Similarly, has anyone tried raising the --min_glue to, say, 10 to improve the accuracy of the assembly?