Dear all,
Im new to this world of seq'g. Right now i have a draft version of a genome (~300 mb) and some RNAseq runs of it from 454.
1. Which is the best software to align RNAseq ? I have us'd newbler and mira. Do you have any more suggestion?
2. How can I compare this both software (newbler and mira) ? To map back these RNA contigs to draft genome, which software I should use?
Your comments are greatly appreciated....
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
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