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  • RNA-seq mapping

    Dear all,
    Im new to this world of seq'g. Right now i have a draft version of a genome (~300 mb) and some RNAseq runs of it from 454.
    1. Which is the best software to align RNAseq ? I have us'd newbler and mira. Do you have any more suggestion?
    2. How can I compare this both software (newbler and mira) ? To map back these RNA contigs to draft genome, which software I should use?

    Your comments are greatly appreciated....
    seq_g

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