Dear fellow sequencers who are much more experienced than I am,
Let's say there's a specific type of mammalian ribosomes and I want to characterize which messages bind to this type. Is anybody aware of any protocol go tackle this?
I would start with a ribosome pellet, but I'm not sure if I would have better to use ribosome protection protocol and analyze the footprints or work from the full length messages?
Any discussion or thoughts would be greatly appreciated!
Let's say there's a specific type of mammalian ribosomes and I want to characterize which messages bind to this type. Is anybody aware of any protocol go tackle this?
I would start with a ribosome pellet, but I'm not sure if I would have better to use ribosome protection protocol and analyze the footprints or work from the full length messages?
Any discussion or thoughts would be greatly appreciated!