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  • konika
    Member
    • Sep 2010
    • 14

    Warnings in cuffdiff subsequently killing the program

    Hi
    I have run the command
    Code:
    cuffdiff -o /wrk/sushilth/analysis/differential_expression/all_samples_redo_cuffmerge2 \
    -b /wrk/sushilth/MM_GFF/Mus_musculus/NCBI/build37.2/Sequence/WholeGenomeFasta/genome.fa \
    -p 8 -L Scr,Lkb1,Nuak1,Nuak2 -u /wrk/sushilth/analysis/merged_asm/merged.gtf \
    /wrk/sushilth/analysis/scr/br1/tophat_out_sbr1/sbr1_accepted_hits.bam,\
    /wrk/sushilth/analysis/scr/br2/tophat_out_sbr2/sbr2_accepted_hits.bam,\
    /wrk/sushilth/analysis/scr/br3/tophat_out_sbr3/sbr3_accepted_hits.bam \
    /wrk/sushilth/analysis/lkb1/br1/tophat_out_lbr1/lbr1_accepted_hits.bam,\
    /wrk/sushilth/analysis/lkb1/br2/tophat_out_lbr2/lbr2_accepted_hits.bam,\
    /wrk/sushilth/analysis/lkb1/br3/tophat_out_lbr3/lbr3_accepted_hits.bam \
    /wrk/sushilth/analysis/nuak1/br1/tophat_out_n1br1/n1br1_accepted_hits.bam,\
    /wrk/sushilth/analysis/nuak1/br2/tophat_out_n1br2/n1br2_accepted_hits.bam,\
    /wrk/sushilth/analysis/nuak1/br3/tophat_out_n1br3/n1br3_accepted_hits.bam \
    /wrk/sushilth/analysis/nuak2/br1/tophat_out_n2br1/n2br1_accepted_hits.bam,\
    /wrk/sushilth/analysis/nuak2/br2/tophat_out_n2br2/n2br2_accepted_hits.bam,\
    /wrk/sushilth/analysis/nuak2/br3/tophat_out_n2br3/n2br3_accepted_hits.bam
    This gives several warnings such as

    Warning: couldn't find fasta record for '13|NT_110857.1'!
    This contig will not be bias corrected.

    And after some processing the program is killed :
    cuffdiff_all_samples_redo.sh: line 26: 35440 Killed

    Maybe anyone can help on what is going wrong.
    Thanks
    Last edited by konika; 02-02-2015, 05:31 AM.

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