Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • arabidopsis
    Member
    • Oct 2010
    • 13

    #1

    Cufflinks: sort order of reads in bams must be the same

    Hi all,

    I am trying to run cufflinks on STAR-aligned reads. After converting to BAM and executing "sort" command I do this:

    cufflinks -uN --GTF /path/to/reference.gtf -b /path/to/genome.fasta --compatible-hit-norm mapped_reads_sorted.bam

    However, cufflinks does not run, reporting the following:
    Error: sort order of reads in bams must be the same

    This is double-confusing, because I am only using one BAM file...

    Can someone help?
  • eieneg
    Junior Member
    • Feb 2017
    • 5

    #2
    Remove COLONS

    Remove colons in the FASTA file and the GFF file.

    Comment

    • eieneg
      Junior Member
      • Feb 2017
      • 5

      #3
      You must remove the colons in the FASTA reference and the GTF file. (make sure the names match up after)

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by SEQadmin2




        NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
        Today, 06:46 AM
      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-28-2026, 09:57 AM
      0 responses
      25 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-28-2026, 09:51 AM
      0 responses
      15 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      71 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      48 views
      0 reactions
      Last Post SEQadmin2  
      Working...