RNA-seq data validation

Caroline Plus

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Join Date: Oct 2015

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#1

RNA-seq data validation

10-24-2015, 08:29 PM

Hi,

How could I validate the results of differentially expressed RNA by the use of RT-qPCR. What positive control should I use- is universal RNA good as a control for salivary RNA? Are other methods for validation of differentially expressed RNA? How to interpret the results of qPCR?

Thanks in advance
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GenoMax

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Join Date: Feb 2008

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10-25-2015, 04:49 AM

Just in case you had not seen this past thread: http://seqanswers.com/forums/showthread.php?t=7814
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RNA-seq data validation

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