Hi all,
I want to do the TMM normalization on my RNA-seq data using EdgeR package in R and have two questions:
1) How can I convert .fastq files to .txt files to be able to feed them into the EdgeR package?
2) My RNA-seq data are paired sequence .fastq files. What quality control should I do on them and how should I merge them together prior to analysis?
Thanks for the help,
Hamid
I want to do the TMM normalization on my RNA-seq data using EdgeR package in R and have two questions:
1) How can I convert .fastq files to .txt files to be able to feed them into the EdgeR package?
2) My RNA-seq data are paired sequence .fastq files. What quality control should I do on them and how should I merge them together prior to analysis?
Thanks for the help,
Hamid
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