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  • nufangdeshengming
    Member
    • Oct 2015
    • 10

    #1

    RNA-seQ trinityrnaseq-2.1.1 different runs get different transcripts!

    sorry,my title is wrong ,i mean that the same run gets the different transcripts!
    I use trinityrnaseq-2.1.1 to assemble the reads to get the transcripts.First i try the following command "bash runMe.sh",using the sample data located in
    trinityrnaseq-2.1.1/sample_data/test_Trinity_Assembly,and i get the result trinity.fasta which is located in the directory trinityrnaseq-2.1.1/sample_data/test_Trinity_Assembly/trinity_out_dir.
    Next,i try the following commands "Trinity -seqType fq -max_memory 2G -left reads.left.fq,reads2.left.fq -right reads.right.fq,reads2.right.fq -SS_lib_type RF -CPU 4",and i get the reasult trinity.fasta.But when i examine the two results above, i find that i get almostly the absolutely different results,i try to search
    on google ,but cannot get the answer,is there some get the same result as mine?
    Last edited by nufangdeshengming; 12-02-2015, 10:35 PM.
  • westerman
    Rick Westerman
    • Jun 2008
    • 1104

    #2
    Well, Trinity is non-deterministic. Because it chooses some running conditions randomly it will not run exactly the same between runs. But the differences should be minor.

    Comment

    • nufangdeshengming
      Member
      • Oct 2015
      • 10

      #3
      But the key point is that it is not just "a few minor differences",there are many differences between them , i donnot what is wrong ,shouldn't the two commonds get almostly the same results?

      Comment

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