Hi friends,
I would like to annotate my de novo transcriptome assembly with KOG /COG database. I'm familar with doing local balstx against nr, uniprot database on linux. But, KOG /COG got me so confused. I read several materials on net, but I don't still know how to make database and do blastx. As far as I found blasting against this database isn't an easy work, at least for me as a biggener. I would be highly appreciate if you would please let me know if there is any web server or tutorial to teach me step-by-step for doing it.
Thanks in advance,
I would like to annotate my de novo transcriptome assembly with KOG /COG database. I'm familar with doing local balstx against nr, uniprot database on linux. But, KOG /COG got me so confused. I read several materials on net, but I don't still know how to make database and do blastx. As far as I found blasting against this database isn't an easy work, at least for me as a biggener. I would be highly appreciate if you would please let me know if there is any web server or tutorial to teach me step-by-step for doing it.
Thanks in advance,