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  • maryam
    Junior Member
    • Apr 2013
    • 8
    #1

    KOG functional annotation

    Hi friends,

    I would like to annotate my de novo transcriptome assembly with KOG /COG database. I'm familar with doing local balstx against nr, uniprot database on linux. But, KOG /COG got me so confused. I read several materials on net, but I don't still know how to make database and do blastx. As far as I found blasting against this database isn't an easy work, at least for me as a biggener. I would be highly appreciate if you would please let me know if there is any web server or tutorial to teach me step-by-step for doing it.

    Thanks in advance,
    Tags: functional annotation, kog, transcriptome annotation
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