TPM and FPLM problem?

Ahmadiut

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Join Date: May 2015

Posts: 3
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TPM and FPLM problem?

06-24-2016, 12:10 AM

Hi
I have used Illumina hiseq 2000 to sequence two samples (two organs of a plant) without replication. I know that without replication I can not deferentially analyze my data and I just want to report the gene expression of each sample (their TPM). The problem is: in one of my sample TPM value of all genes is more than their FPKM and in the other one FPKM>TPM. Is there any problem with my data?
I think my data needs more normalization because TPM values of genes in one of my sample is greater than the other one in most cases.

Example:
Sample1
Genes TPM FPKM
gene1 20 15
gene2 100 85
gene3 250 195

sample2
Genes TPM FPKM
gene1 5 7
gene2 12 20
gene3 35 40

Any help will be appreciated,
Thanks

Last edited by Ahmadiut; 06-24-2016, 04:08 AM.
Tags: differential analysis, normalization, rna-seq, rsem

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