Dear all,
I have a naive question. I would like to do transcriptome sequencing using human total rna as sample. I need to calculate the read depth with this formula: Coverage= (read length*number of reads)/genome size. We all know the human genome size is approximately 3 billions. But do we know the size of the total rna? Am I even asking the right question? Your kind advice would be very much appreciated.
Thank you.
Ed
I have a naive question. I would like to do transcriptome sequencing using human total rna as sample. I need to calculate the read depth with this formula: Coverage= (read length*number of reads)/genome size. We all know the human genome size is approximately 3 billions. But do we know the size of the total rna? Am I even asking the right question? Your kind advice would be very much appreciated.
Thank you.
Ed
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