Hi There,
I am aiming to sequence bacterial genome with NexteraXT 300bp paired-end reads. I have made my library and all the fragments size (including the adapter) are ranging from 200-650 (I check with the bioanalyzer and gel). Do you think that I will have sufficient insert size in the end? Do you think that I will have good insert size for clustering on the MiSeq platform (using Nextera v3 chemistry)?
Would be very helpful of your suggestions here!
Cheers,
Shimul
I am aiming to sequence bacterial genome with NexteraXT 300bp paired-end reads. I have made my library and all the fragments size (including the adapter) are ranging from 200-650 (I check with the bioanalyzer and gel). Do you think that I will have sufficient insert size in the end? Do you think that I will have good insert size for clustering on the MiSeq platform (using Nextera v3 chemistry)?
Would be very helpful of your suggestions here!
Cheers,
Shimul
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