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  • hppRNA: a Snakemake-based handy parameter-free pipeline for RNA-Seq analysis

    Hi there,

    I am delighted to announce the release of a new comprehensive pipeline for RNA-Seq analysis on local server - hppRNA.

    This tool integrates six workflows such as Tophat–Cufflink–Cuffdiff, Subread–featureCounts–DESeq2, STAR–RSEM–EBSeq, Bowtie–eXpress–edgeR, kallisto–sleuth, and HISAT–StringTie–Ballgown to handle fastq files and generate the gene expression matrix and differentially-expressed-genes.

    In addition, it detects SNPs and fusion genes and copes with long noncoding RNAs and circular analysis.

    The link to the package for downloading:

    Download hppRNA for free. A Snakemake-based handy parameter-free pipeline for RNA-Seq analysis. hppRNA package is dedicated to the RNA-Seq analysis for a large number of samples simultaneously from the very beginning to the very end, which is formulated in Snakemake pipeline management system. It starts from fastq files and will produce gene/isoform expression matrix, differentially-expressed-genes, sample clusters as well as detection of SNP and fusion genes by combination of the state-of-the-art software.


    Reference:

    Dapeng Wang. hppRNA-a Snakemake-based handy parameter-free pipeline for RNA-Seq analysis of numerous samples.. Brief Bioinform. 2017 Jan 17. pii: bbw143. doi: 10.1093/bib/bbw143.

    RNA-Seq technology has been gradually becoming a routine approach for characterizing the properties of transcriptome in terms of organisms, cell types and conditions and consequently a big burden has been put on the facet of data analysis, which calls for an easy-to-learn workflow to cope with the i …



    Please let me know if you encounter any issues when using this pipeline. (email address: [email protected])

    Thanks,

    Dapeng
    Last edited by wangdp; 01-24-2017, 10:59 PM.

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