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  • barrmur
    Member
    • Nov 2009
    • 26
    #1

    Sequencing RNA

    Hi all,

    Just wondering if anyone has any experience of the following. I want to sequence a single fragment of RNA to confirm it is what I think it is. Any suggestions on how to go about it. The major problme is it is only 25 bases long. I can convert to DNA and have a 7bp primer but not sure where to go from here. I have access to illumina, pyromark Q24, ABI 3730. I have tried cloning the fragment with no success. Any other suggestions.
    Tags: None
    • malachig
      Senior Member
      • Aug 2010
      • 117
      #2
      Have you considered making a concatamer and then cloning that? Analogous to sequencing of SAGE libraries by Sanger sequencing.

        Comment

        • barrmur
          Member
          • Nov 2009
          • 26
          #3
          Nice idea, might give that a go. Cheers.

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