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10x genomics ans spike-in RNAs

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  • 10x genomics ans spike-in RNAs

    Hello,

    I would like to know whether 10x genomics platforsm add RNA spike-in sequences while doing the single cell RNA-seq? I was not able to find out this information online.

    I saw few genes with Ercc in the gene list, however I am not getting whether they are spike-in RNAs or not. I have asked 10x genomics tech support but there is no reply, How to know whether spike-in RNAs are used or not.

    Your kind help is appreciated.

    Thanks,
    Rashmi

  • #2
    10x Genomics does not use spike-in in their current single-cell RNA-Seq workflow.

    I wonder what gene list you are reffering to.

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