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  • RickC7
    Member
    • Feb 2010
    • 31
    #1

    TruSeq Stranded Total RNA + NEB Barcodes??

    Looking to get more index possibilities out of a TruSeq Stranded Total RNA LT kit. NEB Ultra directional workflow looks the same and NEB will sell the extra barcode kit alone. As far as I can tell, it looks like the NEB index adapters will work with the TruSeq kit, since both use dA-tailing... NEB #E7335, #E7500. Anyone have experience with this?

    Thanks!
    Tags: None
    • MU Core
      Member
      • Apr 2008
      • 60
      #2
      Keep in mind you'll require treatment of the adapter ends with the USER enzyme to free the circularized ends for downstream sequencing chemistry. This is a step you would have to incorporate into the TruSeq workflow. Not sure how easy that would be with the master mixes, etc. of the TruSeq kit.

        Comment

        • RickC7
          Member
          • Feb 2010
          • 31
          #3
          Yes, I found a couple of caveats and work-arounds, but thankfully I won't need to employ any customization as Illumina gratefully offered to send me extra barcodes for this instance.
          Thanks!

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