Hi,
In order to convert the bedgraph file to bigwig file format I need chrom.sizes file. I downloaded pre-built index files from Tophat website to run Tophat. I tried to use bowtie-inspect with -n option on these pre-built index files to create chrom.sizes file, but it generates only chromosome names and not the size of chromosome. Could someone please let me know what other options should I use or if there are any other alternatives to generate this file using pre-built indexes?
Thanks in advance for your responses,
Nirmala
In order to convert the bedgraph file to bigwig file format I need chrom.sizes file. I downloaded pre-built index files from Tophat website to run Tophat. I tried to use bowtie-inspect with -n option on these pre-built index files to create chrom.sizes file, but it generates only chromosome names and not the size of chromosome. Could someone please let me know what other options should I use or if there are any other alternatives to generate this file using pre-built indexes?
Thanks in advance for your responses,
Nirmala