data set from NCBI SRA

[masylichu]

i have downloaded several RNA-seq Data from NCBI SRA, such as SRR019978.fastq.

i want to know whether the raw data has been treated by trimming the adaptor sequence of the RNA-seq. if not, what should i do? and what is the adaptor sequence?

thanks.

[avilella]

I asked this question to the EBI SRA people, and I was told that both NCBI and EBI SRA apply a series of QC procedures before they store the sequences. If you see any noise in the data, like adaptors, please report back to them, because it shouldn't be there.

[VC87]

hi!does anyone knows how to search sra files from Bs seq library fixing metadata features such as organism, tissue, age etc..?thanks