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  • SamH2017
    Junior Member
    • Sep 2017
    • 2

    #1

    Hisat2: no command found in bash

    Hi all,
    I'm a complete novice at RNAseq mapping and I am trying to follow this protocol using their data: https://www.nature.com/nprot/journal...t.2016.095.pdf

    I have downloaded the appropriate files and unpacked them and copied the relevant files into my working directory (HOME/bin) but when I try to run the first command to map the reads to the genome ($ hisat2 -p 8 --dta -x chrX_data/indexes/chrX_tran -1) bash spits out the following error: -bash: hisat2: command not found

    The hisat2 executable file in present in my working directory, so I can't understand why it won't run.
    Any advice would be much appreciated as my boss is back next week and I would like to have made some progress by then. I've been struggling with this since yesterday and can't seem to get past it.

    Thank you in advance.
    Samantha
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    While you would be able to get the command to run by doing
    Code:
    $ ./hisat2 -p 8 --dta -x chrX_data/indexes/chrX_tran -1 etc
    you should spend a bit of time with some unix basics. I recommend this UNIX tutorial.

    Comment

    • SamH2017
      Junior Member
      • Sep 2017
      • 2

      #3
      Problem solved!

      Thank you so much GenoMax!! This advice worked a treat!

      I will definitely take the time to learn the basics, and hopefully beyond! Thanks for the tutorial link.

      Comment

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