Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • SamH2017
    Junior Member
    • Sep 2017
    • 2

    Hisat2: no command found in bash

    Hi all,
    I'm a complete novice at RNAseq mapping and I am trying to follow this protocol using their data: https://www.nature.com/nprot/journal...t.2016.095.pdf

    I have downloaded the appropriate files and unpacked them and copied the relevant files into my working directory (HOME/bin) but when I try to run the first command to map the reads to the genome ($ hisat2 -p 8 --dta -x chrX_data/indexes/chrX_tran -1) bash spits out the following error: -bash: hisat2: command not found

    The hisat2 executable file in present in my working directory, so I can't understand why it won't run.
    Any advice would be much appreciated as my boss is back next week and I would like to have made some progress by then. I've been struggling with this since yesterday and can't seem to get past it.

    Thank you in advance.
    Samantha
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    While you would be able to get the command to run by doing
    Code:
    $ ./hisat2 -p 8 --dta -x chrX_data/indexes/chrX_tran -1 etc
    you should spend a bit of time with some unix basics. I recommend this UNIX tutorial.

    Comment

    • SamH2017
      Junior Member
      • Sep 2017
      • 2

      #3
      Problem solved!

      Thank you so much GenoMax!! This advice worked a treat!

      I will definitely take the time to learn the basics, and hopefully beyond! Thanks for the tutorial link.

      Comment

      Latest Articles

      Collapse

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 06-09-2026, 11:58 AM
      0 responses
      23 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-05-2026, 10:09 AM
      0 responses
      29 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-04-2026, 08:59 AM
      0 responses
      39 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-02-2026, 12:03 PM
      0 responses
      61 views
      0 reactions
      Last Post SEQadmin2  
      Working...