Hello,
I would like to detect lncRNAs from RNA-seq data. Initially, I'm using Hisat2 for aligning the samples. For this I downloaded the human genome hisat2 index "genome_snp_tran" from the hisat2 webiste. [https://ccb.jhu.edu/software/hisat2/manual.shtml]
I would like to know whether this "genome_snp_tran" is built with splice sites already?
Why splice sites are needed? If there are necessary and if the genome index I downloaded is not built with splice sites how can I get the splice sites?
thankyou
bvk
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Differential Expression and Data Visualization: Recommended Tools for Next-Level Sequencing Analysis
After covering QC and alignment tools in the first segment and variant analysis and genome assembly in the second segment, we’re wrapping up with a discussion about tools for differential gene expression analysis and data visualization. In this article, we include recommendations from the following experts: Dr. Mark Ziemann, Senior Lecturer in Biotechnology and Bioinformatics, Deakin University; Dr. Medhat Mahmoud Postdoctoral Research Fellow at Baylor College of Medicine;...05-23-2023, 12:26 PM -
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