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NORA: Accuracy approximates RSEM+bowtie2 with the speed of Kallisto and Salmon
Introduction
We introduce Nora, a tool for transcript quantification where accuracy matters. Nora is much more accurate than Hera, an algorithm that we released last year (Hera obtained the best ranking in the latest round of SMC-RNA DREAM challenge). Nora also outputs accurate read alignments in bam format, while time and memory consumption is similar to pseudo-alignment approaches’.
Nora source code is written in C using the linux kernel coding style . The compiled package is tiny: 262 KB, and can be installed on most macOS and linux distributions without dependencies. For now, Nora’s binary package is not yet released for public. To use Nora, you need to get an invitation.
Benchmark Data
We benchmarked Nora and other transcript quantification tools (Hera, Kallisto, Salmon, RSEM+Bowtie2) using 20 simulated data sets generated from Kallisto paper (using this script), and the most recent benchmark data from SMC-RNA DREAM challenge.
Benchmark Results
Please visit the homepage of Nora for full of the benchmark tables. https://bioturing.com/nora
Try Nora
Nora will soon be available for both Linux and Mac in a command line interface, a lite app version, a workstation platform, and a cloud application. If you are interested in Nora and want to give it a try, just let us know through [email protected].
Thank you!
Introduction
We introduce Nora, a tool for transcript quantification where accuracy matters. Nora is much more accurate than Hera, an algorithm that we released last year (Hera obtained the best ranking in the latest round of SMC-RNA DREAM challenge). Nora also outputs accurate read alignments in bam format, while time and memory consumption is similar to pseudo-alignment approaches’.
Nora source code is written in C using the linux kernel coding style . The compiled package is tiny: 262 KB, and can be installed on most macOS and linux distributions without dependencies. For now, Nora’s binary package is not yet released for public. To use Nora, you need to get an invitation.
Benchmark Data
We benchmarked Nora and other transcript quantification tools (Hera, Kallisto, Salmon, RSEM+Bowtie2) using 20 simulated data sets generated from Kallisto paper (using this script), and the most recent benchmark data from SMC-RNA DREAM challenge.
Benchmark Results
Please visit the homepage of Nora for full of the benchmark tables. https://bioturing.com/nora
Try Nora
Nora will soon be available for both Linux and Mac in a command line interface, a lite app version, a workstation platform, and a cloud application. If you are interested in Nora and want to give it a try, just let us know through [email protected].
Thank you!
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