Dear All,
After a lot of trouble with getting my Input file correct for Trans-abyss, I am unable to understand why does it require a Reference genome to run? It uses mm9 (mouse) or hg18 (human) only as reference.
Why in the first place will I go for trans-abyss if I had a reference genome? It is an RNA-seq data, on which I have already executed ABySS- it produced contigs.fa.
Why is this contigs.fa not being used as an input file directly to generate transcripts?
Any insights on these two queries will help me a lot to understand the tool better!!
Thanks in advance..
After a lot of trouble with getting my Input file correct for Trans-abyss, I am unable to understand why does it require a Reference genome to run? It uses mm9 (mouse) or hg18 (human) only as reference.
Why in the first place will I go for trans-abyss if I had a reference genome? It is an RNA-seq data, on which I have already executed ABySS- it produced contigs.fa.
Why is this contigs.fa not being used as an input file directly to generate transcripts?
Any insights on these two queries will help me a lot to understand the tool better!!
Thanks in advance..
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