Dear All,
I have read in a lot of forums as well as discussions posted on the web, which say that the way coverage is calculated for genome, is not the way we can get for transcriptome data as the transcripts level will change from sample to sample. I agree to this.
All I want to knw is If my client says they have given us 20x coverage in RNA-seq data for a particular organism, then how can I reconfirm that the coverage is really 20x? Is there anyway to do this?
Any insight on this will be help me a lot...
Thanks in advance..
I have read in a lot of forums as well as discussions posted on the web, which say that the way coverage is calculated for genome, is not the way we can get for transcriptome data as the transcripts level will change from sample to sample. I agree to this.
All I want to knw is If my client says they have given us 20x coverage in RNA-seq data for a particular organism, then how can I reconfirm that the coverage is really 20x? Is there anyway to do this?
Any insight on this will be help me a lot...
Thanks in advance..
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