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Transcriptome construction
Hello all
I wanted to ask that whether i can use both genome and transcriptome (generated using PacBio) as reference together and align Illumina RNA-Seq PE reads using HISAT2.
I want to use both because genome assembly is not good and many PacBio transcripts are not aligning to genome. Also alignment percentage for few samples is very low to genome.
Thank you
I wanted to ask that whether i can use both genome and transcriptome (generated using PacBio) as reference together and align Illumina RNA-Seq PE reads using HISAT2.
I want to use both because genome assembly is not good and many PacBio transcripts are not aligning to genome. Also alignment percentage for few samples is very low to genome.
Thank you